ENZO熱銷產(chǎn)品——ER-ID® Green assay kit內(nèi)質(zhì)網(wǎng)檢測試劑盒（綠色）

Enzo Life Sciences公司的ER-ID® Green assay kit適用于活細(xì)胞或破膜醛固定細(xì)胞的染色。微摩爾級濃度的ER-ID®綠色染料足以對哺乳動物細(xì)胞進(jìn)行染色，在人宮頸癌細(xì)胞系、T淋巴細(xì)胞系、Jurkat 細(xì)胞系、HeLa 細(xì)胞系以及骨肉瘤上皮細(xì)胞U2-OS中已經(jīng)得到了驗(yàn)證。ER-ID® Green assay kit專門用于表達(dá)RFP或OFP的細(xì)胞系，以及表達(dá)藍(lán)色或青色熒光蛋白（BFP、CFP）的細(xì)胞。該試劑盒適用于活細(xì)胞或固定后細(xì)胞與探針（如標(biāo)記抗體）或與Texas Red或香-豆-素類似光譜性質(zhì)的其他熒光綴合物結(jié)合使用。

產(chǎn)品特點(diǎn)

● 新型內(nèi)質(zhì)網(wǎng)染料，可用于活細(xì)胞、破膜細(xì)胞或固定細(xì)胞的染色

● 與常見熒光染料和熒光蛋白兼容

● 高耐光漂白性、抗?jié)舛却銣绾凸廪D(zhuǎn)換

● 生產(chǎn)嚴(yán)格，以控制和消除非特異性結(jié)果

如需購買Enzo Life Sciences公司產(chǎn)品，或咨詢產(chǎn)品技術(shù)問題，請聯(lián)系代理商欣博盛生物。

實(shí)例分析

● ER-ID®綠色染色與含有KDEL序列的鈣網(wǎng)蛋白共定位，與橙色熒光蛋白（OFP）融合。

● 用ER-ID®綠色染料（A）、Hoechst 33342染料（B）對HeLa活細(xì)胞進(jìn)行染色，得到的合成圖像（C）。

● 用4%多聚甲醛固定HepG2細(xì)胞20分鐘，并用ER-ID®標(biāo)記30分鐘。下圖為標(biāo)記3天后拍攝的照片。

● 將HepG2細(xì)胞與ER-ID®孵育30分鐘，并用倒置顯微鏡（Zeiss）進(jìn)行分析。

產(chǎn)品詳情：

部分產(chǎn)品文獻(xiàn)引用

1. Molecular interplay between NOX1 and autophagy in cadmium-induced prostate carcinogenesis: A. Tyagi, et al.; Free Radic. Biol. Med. 199, 44 (2023) 

2. Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum: A. Komatsu, et al.; J. Biol. Chem. 298, 101933 (2022) 

3. PLP1 mutations in patients with multiple sclerosis: identification of a new mutation and potential pathogenicity of the mutations: N.C. Cloake, et al.; J. Clin. Med. 7, 342 (2018), Application(s): Fluorescence microscopy 

4. Englerin A induces an acute inflammatory response and reveals lipid metabolism and ER stress as targetable vulnerabilities in renal cell carcinoma: A. Batova, et al.; PLoS One 12, e0172632 (2017), Application(s): Fluorescence microscopy

5. Cell growth on (“Janus") density gradients of bifunctional zeolite L crystals: N.S. Kehr, et al.; ACS Appl. Mater. Interfaces 8, 35081 (2016)

6. The parathyroid hormone second receptor PTH2R and its ligand tuberoinfundibular peptide of 39 residues TIP39 regulate intracellular calcium and influence keratinocyte differentiation: E. Sato, et al.; J. Invest. Dermatol. 136, 1449 (2016)

7. Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies: V. Ramanan, et al.; Virology 494, 236 (2016), Application(s): Endoplasmic reticulum staining

8. Localization-Dependent Cell-Killing Effects of Protoporphyrin (PPIX)-Lipid Micelles and Liposomes in Photodynamic Therapy: S. Tachikawa, et al.; Bioorg. Med. Chem. 23, 7578 (2015), Application(s): Cell staining

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